Fig.3 AgNOR staining with peripheral blood lymphocytes. . The technical aspect of AgNOR staining procedure should be carefully dealt with. Attention should. Conclusions: AgNOR staining can be considered as a useful adjunct to adjunct procedures which can diagnose malignancy at the earliest. Silver staining results are comparable to those obtained. [A standardized AgNOR stain method for formalin fixed and paraffin embedded .. to be a good adjunct to presently available frozen section techniques and cytology.

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In previous methods counter stain Neutral red was used.

We recommend the use of morphometry for AgNOR size in future. Oil can be removed with xylene. A histopathological study on bone marrow trephine biopsies. The AgNOR dots have become blurred due to dim background and less clearly visible as compared to photomicrograph a. However, AgNORs seem to reflect proliferation independent of cellular and nucleolar activity of tumour cells, as well Bankfalvi et al.

AgNOR number and distribution in the nucleus configuration were useful in the detection and prognosis of some neoplasias, such as renal, bladder, and pharyngeal carcinoma, multiple myeloma, and skin melanocytic lesions Pich et al. The tissue was then incubated in acid alcohol three parts ethanol: The sections were then washed in distilled water, dehydrated in graded alcohol and then xylene and cover slipped.

The AgNOR dots were brighter and more clear with modified staining when compared with previous studies. AgNOR size and dispersion may be more important than counting alone: Cancer Genet Cytogenet 35, Abstract This study was conducted in the department of Pathology King Edward Medical University, from June to December to introduce the new method of AgNOR staining and its interpretation to increase its reliability.


This study was conducted in the department of Pathology King Edward Medical University, from June to December to introduce the new method of AgNOR staining and its interpretation to increase its reliability.

It is advisable to wear gloves as the black spots are not very attractive. It was helpful in the classification of urothelial carcinoma in different grades of malignancy and in subdividing patients with grade II transitional cell carcinomas into low- and high-risk groups Helpap et al.

We conclude that modified AgNOR staining technique is simple, quick and reliable to evaluate cell proliferation by detecting AgNORs size and dispersion. In situ Hybridization Methods protocols.

AgNOR staining and quantification.

Quantity of procedur silver-stained proteins is related to proliferative activity in cancer cells. Small-capped bottles were used in which different quantities of silver nitrate like 1, 2, 3 or 4 g were porcedure. AgNORs can be identified as black dots in the nuclei. If necessary apply another drop of silver nitrate solution on top. After silver-staining, the NORs can be easily identified as black dots exclusively localised throughout the nucleolar area, and are called “AgNORs”.

Interphase nucleolar organizer regions in cancer cells. Received Aug 12; Accepted Nov Re-apply xylene if it dries out. Incubate in a moist chamber at C until golden brown about h.

It was concluded, that standardized AgNOR parameters correlate with markers of increased malignant potential in breast carcinomas. Our results are in accordance with studies on bladder carcinoma and multiple myeloma showing association between mean AgNOR count and histological grade of differentiation Pich et al.


Modified method of AgNOR staining for tissue and interpretation in histopathology

Indeed, the absolute AgNOR scores reported by different authors for the same types of tumour are scarcely comparable and the results produced by these investigations sometimes seem to be conflicting. The mean dispersion of AgNOR per cell in normal tissue and reactive gliosis was nil, while low in low-grade astrocytoma and high in procesure astrocytoma mean 1.

The results are in accordance with previous reports in multiple myeloma Pich et al. These findings are not consistent with those reported by Mourad et al. The grading of dots dispersion was performed according to Khan et al. Moreover, in cancer tissues AgNOR protein expression was wtaining to be strictly related to the cell duplication rate.

AgNOR staining and quantification.

The tissue was then ready for counts. The nuclei stain light yellow and out line of nuclei as well as cells were usually clearly visible. However, the lack of a standardised silver-staining protocol has led to much misinterpretation of ataining structures evaluated in individual studies.