CHARDAKOV METHOD PDF

2) In “Chardakov method for water potential measurement” experiment, you find that M solution has not been changed by water loss or absorption by the. Chardakov Technique. Plant Physiology. UNI. Incubating tissue in solution. Pre- incubation. Incubation. Post-incubation. Save for later measurement. Did solution change concentration? Solutes stay the same If water left or entered tissue –Water also entered or left solution –Solution becomes more dilute or.

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Cytology Osmosis and Water relations. A measure of how much material is packed into a unit volume of the material The fewer.

Chardakov method Essay – Free Papers and Essays Examples

Cut them to the same length ca. Weigh the cores to the nearest 0.

Work quickly to minimize evaporation and keep the tissue wrapped in a moist towel. Then, the percent change in weight of the tissue is plotted versus solution concentration or osmotic potential. Does a gas differ from other fluids?

It is assumed that solute movement between tissue and solution is negligible. If you wish to download it, please recommend it to your friends in any social system.

Chardakov method

Dispense 10 chardako of water or a sucrose solution 0. What is the water potential calculated by this method? The answer will be in Jm -3 which are equivalent to pressure Pa. OK Any substance that can change shape or flow easily.

Which is more accurate? In contrast to the Chardakov method which analyzes changes in solution density after incubation, this technique monitors tissue weight changes. Draw the best fit line for your data.

Feedback Privacy Policy Feedback. In both techniques, tissue samples are incubated in a series of solutions of known osmotic water potential. Record the temperature of the solutions Table 1 Using a Pasteur pipet, remove a small amount methld water dyed with methylene blue charfakov dye the sucrose solution, dip a dry probe into methylene blue methov and then mix.

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Hypertonic Concentration with higher solute concentration and less water concentration Hypotonic chardaiov solute concentration and more water concentration. Dispense 10 mL of water or sucrose 0. Record your data in Table 2. What would happen to The size of the tissue? Incubate the cores for at least 1. The water potential of the tissue is considered to be equal the osmotic potential of the incubating solution at which there is no change in tissue weight i.

Weigh two or preferably three cores, record your data in Table 2 and then place the cores in one of chsrdakov test solutions. Pour off the solutions into a set of empty, correspondingly labeled tubes. Use the following equation to calculate the percent change in weight for each tissue by the following equation: The water potential measured by this technique should be the same as that obtained the Chardakov method.

Incubate the cores for 1. Chardakov Method for Determining Water Potential.

This method depends on the change in density in methid solution that occurs after a tissue has been immersed in it. Measuring Water Potential by the Gravimetric Technique. One distinct advantage of this technique is that it provides a more accurate estimate of water potential.

Immerse the pipette in the water that previously had tissue sections in it until the tip is approximately at the center of the tube.

My presentations Profile Feedback Log out. Complete Tables 1 and 2. Does it correspond to the value obtained by the Chardakov technique?

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Published by Sabrina Grant Modified over chardaklv years ago. Response of drops float, sink, hover when placed in solutions in which potato cores have been incubated.

Periodically swirl the containers. A “kink” may be observed in the graph below the x-axis. In this method, tissue samples are weighed before and after incubation in a series of solutions of known osmotic water potential.

Plant Physiology

The Chardokov method provides a quick means to determine plant tissue water potentials. Do the cores show various degrees of turgor? Record your results Table 2 and repeat this procedure for each of the sucrose solutions.

Use a cork borer to prepare at least 27 uniform tissue samples from the potato. This technique for measuring water potential is similar in theory to the Chardakov method and shares the advantage cuardakov being simple to perform and doesn’t require expensive equipment. Incubating tissue in solution Pre-incubationIncubation Post-incubation Save for later measurement.

To use this website, you must agree to our Privacy Policyincluding cookie policy. Use a cork borer to prepare at least 27 uniform tissue samples from the potato. Mix the tubes thoroughly with a vortex cbardakov. Alternately, for a more accurate measurement of changes in the solution density, a refractometer can be used.

Repeat for all solutions.

This occurs when the apoplast becomes infiltrated with water and solutes. From the graph, determine the concentration of the sucrose solution in which there was no net weight gain i.